Temperature: Each enzyme works best at certain temperature,it is called Optimum temperature.The enzyme action rate increases when it gets closer to optimum temperature but if temperature is lowered,the rate of activity decreases
Ph degree:Same in temperature enzymes work best at certain ph degrees
Outside Factors:heavy metal ions, antibiotics, arsenic, snake or scorpion poisons act as inhibitors and inhibit enzyme action,on the other hand Some substances increase the activity of an enzyme called as activators for example Magnesium.
Amount of enzymes: If the amount of enzyme is increased, while the substrate remains constant the rate of reaction is also increased. This increase continues up to a point, and then remains at that level. If the concentration of substrate increases continuously, then, the rate of reaction will also increase directly proportional with the enzyme concentration.
Amount of substrate: Adding more substrate will increase the rate of reaction until all enzyme substrate molecules are in action. Adding more substrate will not increase the reaction rate at this point.
